Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats. α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. Prepare cDNA for RT-qPCR directly from fewer than 1,000 cellsĬellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. Perform 1-step real-time RT-PCR with TB Green PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR037A and RR037B)Īvoid genomic DNA contamination during cDNA synthesis and RT-qPCR PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. Make cDNA using a kit that provides RTase and primers as separate components PrimeScript RT Master Mix (Perfect Real Time) (Cat. Make cDNA using a master mix that includes RTase and primers PrimeScript reverse transcriptase is engineered to allow full-length cDNA synthesis reactions at lower temperatures (e.g., 42☌), decreasing the risk of RNA degradation that occurs during conventional reactions performed at higher temperatures.įirst-strand cDNA synthesis for real-time RT-PCR (qPCR)Ĭhoose the right PrimeScript Kit for Real-Time RT-PCR: If you want to.
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PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) enzyme and is verified to be RNase H-minus. PrimeScript RTase enables reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure, making this real-time RT-PCR kit ideal for both routine applications and challenging reverse transcriptase reactions alike. The PrimeScript recombinant RTase enzyme is robust, versatile, and well-suited for applications requiring full-length cDNA synthesis. This real-time RT-PCR kit includes PrimeScript reverse transcriptase, which has exceptionally strong strand-displacement activity for optimal efficiency during cDNA synthesis. Use it upstream of a TB Green- or probe-based qPCR premix from Takara Bio, such as TB Green Premix Ex Taq II (Tli RNase H), TB Green Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).
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This kit is designed for two-step real-time RT-PCR. Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase inhibitor, random 6mers, oligo dT primer, dNTPs, and reaction buffer). cDNA synthesis reactions are easy to assemble-just add RNA template and water. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. To minimize sample-handling time, look no further than this real-time RT-PCR kit.